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guinea pig anti vglut3  (Synaptic Systems)


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    Structured Review

    Synaptic Systems guinea pig anti vglut3
    Guinea Pig Anti Vglut3, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guinea+pig+anti-vglut3/135+204/pm42637985-58-36-39
    Average 93 stars, based on 24 article reviews
    guinea pig anti vglut3 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Immunohistochemistry:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Immunolabeling:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Immunofluorescence:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Fluorescence:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Mass Spectrometry:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Membrane:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Marker:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Isolation:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Transformation Assay:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Expressing:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Western Blot:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Protein Enrichment:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Binding Assay:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Functional Assay:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Incubation:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    In Situ Hybridization:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Plasmid Preparation:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Injection:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Immunostaining:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).

    Staining:

    Article Title: Evidence for a role of 5-HT-glutamate co-releasing neurons in acute stress mechanisms
    Article Snippet: In brief, sections were incubated overnight with the following primary antibodies: rabbit anti-c-Fos (1:1000, Abcam), goat anti-TPH2 (1:1000, Abcam), guinea pig anti-vGLUT3 (1:500 dilution, Synaptic Systems).

    Article Title: C-LTMRs Regulate Thermosensation and Gate the Transition from Acute to Chronic Pain
    Article Snippet: The primary antibodies used in this study were chicken anti-GFP (1:1000, Thermo Fisher Scientific, A10262); rabbit anti-P2X3 (1:1000 Neuromics Cat# RA10109, RRID: AB_2157931 ); rabbit anti-CGRP (1:1000, Cabiochem, PC205L, for DRG staining); rabbit anti-neurofilament M (145 kDa) (1:1000, Sigma-Aldrich AB1987); rabbit anti-TH (1:500, Sigma-Aldrich AB152); rat anti-TAFA4 (1:2000, a gift from Sophie Ugolini (CIML)); goat anti-TRKC (1:500, R and D Systems Cat# AF1404, RRID: AB_2155412 ); guinea pig anti-VGLUT3 (1:1000, Synaptic Systems, 135204).

    Article Title: VGluT3 BNST neurons transmit GABA and restrict sucrose consumption.
    Article Snippet: For Ai193/VGluT3::Cre/VGaT::Flp mice, the primary antibodies were goat anti-ChAT (Invitrogen PA518518), rabbit anti-tdTomato (Takara 632496), and mouse anti-GFP (Takara JL8).

    Article Title: Parallel streams of raphe VGLUT3-positive inputs target the dorsal and ventral hippocampus in each hemisphere
    Article Snippet: Sections were then incubated with the following primary antibodies for 48 h at 4 °C: chicken anti-GFP (1:1000, Invitrogen cat. No. A10262), rabbit anti-RFP (1:10000, VWR Rockland cat. No. CA600-401-379), guinea pig anti-Tph2 (1:1000, Frontier Institute cat. No. TPH2-GP-Af900) or guinea pig anti-VGLUT3 (1:1000, Synaptic Systems cat. No. 135204).

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells
    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).


    Article Title: Serotonergic Modulation of the BNST-CeA Circuit Promotes Sex Differences in Fear Learning
    Article Snippet: Sections were incubated in rabbit anti-EYFP (1:500, ThermoFisher Scientific, #A11122), mouse anti-GAD65/67 (1:250, Santa Cruz Biotechnology, #sc-365180), and guinea pig anti-vGlut3 (1:250, Synaptic Systems, #135204) in 1% normal donkey serum in PBST for 48 hours at 4°C.

    Article Title: Increased c-Fos immunoreactivity in anxiety-related brain regions following paroxetine discontinuation.
    Article Snippet: Following initial analyses of TPH2 immunoreactivity in the DRN, further sections containing the ventral DRN were triple-labelled for c-Fos, TPH2 and VGLUT3 immunoreactivity (primary antibodies: c-Fos and TPH2 as above; guinea pig anti-VGLUT3 [Synaptic Systems, 135 204], 1:500; secondary antibodies at 1:1000: donkey anti-rabbit as above; donkey antigoat IgG H&L Alexa Fluor® 647 [Abcam, ab150131]; CyTM3 AffiniPure goat anti-guinea Pig IgG (H+L) Conjugate [Jackson Immune Research, 106-165-003]).



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